Cloning of the RNA8 gene of Saccharomyces cerevisiae, detection of the RNA8 protein, and demonstration that it is essential for nuclear pre-mRNA splicing.

Strains of Saccharomyces cerevisiae that bear the temperature-sensitive mutation rna8-1 are defective in nuclear pre-mRNA splicing at the restrictive temperature (36 degrees C), suggesting that the RNA8 gene encodes a component of the splicing machinery. The RNA8 gene was cloned by complementation o...

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Udgivet i:Mol Cell Biol
Main Authors: Jackson, S P, Lossky, M, Beggs, J D
Format: Artigo
Sprog:Inglês
Udgivet: Taylor & Francis 1988
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC363249/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2835658/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.8.3.1067
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