Synthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domain.

We have used an in vitro transcription and translation system to synthesize an enzymatically active FLP protein. The FLP mRNA synthesized in vitro by SP6 polymerase is translated efficiently in a rabbit reticulocyte lysate to produce enzymatically active FLP. Using this system, we assessed the effec...

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Bibliografiska uppgifter

I publikationen:	Mol Cell Biol
Huvudupphov:	Amin, A A, Sadowski, P D
Materialtyp:	Artigo
Språk:	Inglês
Utgiven:	Taylor & Francis 1989
Ämnen:	Research Article
Länkar:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362991/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2664465/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.5.1987-1995.1989
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