Synthesis of an enzymatically active FLP recombinase in vitro: search for a DNA-binding domain.
We have used an in vitro transcription and translation system to synthesize an enzymatically active FLP protein. The FLP mRNA synthesized in vitro by SP6 polymerase is translated efficiently in a rabbit reticulocyte lysate to produce enzymatically active FLP. Using this system, we assessed the effec...
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| Publicat a: | Mol Cell Biol |
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| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1989
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362991/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2664465/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.9.5.1987-1995.1989 |
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