Raman Spectra of Interchanging β lactamase Inhibitor Intermediates on the Millisecond Time Scale

Rapid mix - rapid freeze is a powerful method to study the mechanisms of enzyme-substrate reactions in solution. Here we report a protocol that combines this method with normal (non-resonance) Raman microscopy to enable us to define molecular details of intermediates at early time points. With this...

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Detalhes bibliográficos
Main Authors: Torkabadi, Hossein Heidari, Che, Tao, Shou, Jingjing, Shanmugam, Sivaprakash, Crowder, Michael W., Bonomo, Robert A., Pusztai-Carey, Marianne, Carey, Paul R.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3624989/
https://ncbi.nlm.nih.gov/pubmed/23406484
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja311440p
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