A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity.
We describe two retroviral vector-based recombination substrate systems designed to assay for lymphoid VDJ recombinase activity in cultured cells. Both substrates incorporate a constitutive dominant marker gene (the simian virus promoter-driven neo gene) to allow selection of cells that stably integ...
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| Опубліковано в:: | Mol Cell Biol |
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| Автори: | , , , , , , |
| Формат: | Artigo |
| Мова: | Inglês |
| Опубліковано: |
Taylor & Francis
1990
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| Предмети: | |
| Онлайн доступ: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2320007/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.4.1697-1704.1990 |
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