A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity.

We describe two retroviral vector-based recombination substrate systems designed to assay for lymphoid VDJ recombinase activity in cultured cells. Both substrates incorporate a constitutive dominant marker gene (the simian virus promoter-driven neo gene) to allow selection of cells that stably integ...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Yancopoulos, G D, Nolan, G P, Pollock, R, Prockop, S, Li, S C, Herzenberg, L A, Alt, F W
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC362275/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2320007/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.4.1697-1704.1990
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A novel fluorescence-based system for assaying and separating live cells according to VDJ recombinase activity.

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