Stable, resealable pores formed in sea urchin eggs by electric discharge (electroporation) permit substrate loading for assay of enzymes in vivo.
We describe a simple electroporation procedure for loading suspensions of unfertilized sea urchin eggs with impermeant small molecules under conditions that allow close to 90% successful fertilization and development. Poration is carried out in a low-Ca2+ medium that mimicks the intracellular milieu...
Guardado en:
| Publicado en: | Cell Regul |
|---|---|
| Autores principales: | , |
| Formato: | Artigo |
| Lenguaje: | Inglês |
| Publicado: |
American Society for Cell Biology
1989
|
| Materias: | |
| Acceso en línea: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC361426/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2519619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1091/mbc.1.1.65 |
| Etiquetas: |
Sin Etiquetas, Sea el primero en etiquetar este registro!
|