Stable, resealable pores formed in sea urchin eggs by electric discharge (electroporation) permit substrate loading for assay of enzymes in vivo.
We describe a simple electroporation procedure for loading suspensions of unfertilized sea urchin eggs with impermeant small molecules under conditions that allow close to 90% successful fertilization and development. Poration is carried out in a low-Ca2+ medium that mimicks the intracellular milieu...
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| Udgivet i: | Cell Regul |
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| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
American Society for Cell Biology
1989
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC361426/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2519619/ https://ncbi.nlm.nih.govhttps://doi.org/10.1091/mbc.1.1.65 |
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