Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.

A cDNA encoding Cryptococcus neoformans orotidine monophosphate pyrophosphorylase (OMPPase) has been isolated by complementation of the cognate Escherichia coli pyrE mutant. The cDNA was used as a probe to isolate a genomic DNA fragment encoding the OMPPase gene (URA5). By using electroporation for...

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Veröffentlicht in:	Mol Cell Biol
Hauptverfasser:	Edman, J C, Kwon-Chung, K J
Format:	Artigo
Sprache:	Inglês
Veröffentlicht:	Taylor & Francis 1990
Schlagworte:	Research Article
Online-Zugang:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC361041/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2201894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.9.4538-4544.1990
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