Isolation of the URA5 gene from Cryptococcus neoformans var. neoformans and its use as a selective marker for transformation.
A cDNA encoding Cryptococcus neoformans orotidine monophosphate pyrophosphorylase (OMPPase) has been isolated by complementation of the cognate Escherichia coli pyrE mutant. The cDNA was used as a probe to isolate a genomic DNA fragment encoding the OMPPase gene (URA5). By using electroporation for...
Guardat en:
| Publicat a: | Mol Cell Biol |
|---|---|
| Autors principals: | , |
| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
Taylor & Francis
1990
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC361041/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2201894/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.9.4538-4544.1990 |
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