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Conditional mutations occur predominantly in highly conserved residues of RNA polymerase II subunits.

Conditional mutations in the Saccharomyces cerevisiae RNA polymerase II large subunit, RPB1, were obtained by introducing a mutagenized RPB1 plasmid into yeast cells, selecting for loss of the wild-type RPB1 gene, and screening the cells for heat or cold sensitivity. Sequence analysis of 10 conditio...

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Published in:Mol Cell Biol
Main Authors: Scafe, C, Martin, C, Nonet, M, Podos, S, Okamura, S, Young, R A
Format: Artigo
Language:Inglês
Published: Taylor & Francis 1990
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Research Article
Online Access:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC361019/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2406567/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.3.1270-1275.1990
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