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Rapid expression screening of eukaryotic membrane proteins in Pichia pastoris

The overexpression of milligram quantities of protein remains a key bottleneck in membrane protein structural biology. A challenge of particular difficulty has been the overproduction of eukaryotic membrane proteins. In order to cope with the frequently poor expression levels associated with these c...

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Detalhes bibliográficos
Main Authors: Brooks, Cory L, Morrison, Melissa, Joanne Lemieux, M
Formato: Artigo
Idioma:Inglês
Publicado em: Wiley Subscription Services, Inc., A Wiley Company 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3610048/
https://ncbi.nlm.nih.gov/pubmed/23339074
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1002/pro.2223
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