In vivo analysis of the Saccharomyces cerevisiae HO nuclease recognition site by site-directed mutagenesis.

HO nuclease introduces a specific double-strand break in the mating-type locus (MAT) of Saccharomyces cerevisiae, initiating mating-type interconversion. To define the sequence recognized by HO nuclease, random mutations were produced in a 30-base-pair region homologous to either MAT alpha or MATa b...

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Bibliographic Details

Published in:	Mol Cell Biol
Main Authors:	Nickoloff, J A, Singer, J D, Heffron, F
Format:	Artigo
Language:	Inglês
Published:	Taylor & Francis 1990
Subjects:	Research Article
Online Access:	https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360989/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2406563/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.3.1174-1179.1990
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