In vivo analysis of the Saccharomyces cerevisiae HO nuclease recognition site by site-directed mutagenesis.
HO nuclease introduces a specific double-strand break in the mating-type locus (MAT) of Saccharomyces cerevisiae, initiating mating-type interconversion. To define the sequence recognized by HO nuclease, random mutations were produced in a 30-base-pair region homologous to either MAT alpha or MATa b...
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| Опубликовано в:: | Mol Cell Biol |
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| Главные авторы: | , , |
| Формат: | Artigo |
| Язык: | Inglês |
| Опубликовано: |
Taylor & Francis
1990
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| Предметы: | |
| Online-ссылка: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360989/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2406563/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.3.1174-1179.1990 |
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