Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

To delineate segments of the genome of the human protozoan parasite Leishmania major necessary for replication and expression, we developed a vector (pR-NEO) which can be reproducibly introduced into L. major. This DNA was derived from a 30-kilobase extrachromosomal amplified DNA bearing the dihydro...

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Autores principales: Kapler, G M, Coburn, C M, Beverley, S M
Formato: Artigo
Lenguaje:Inglês
Publicado: 1990
Materias:
Research Article
Acceso en línea:https://ncbi.nlm.nih.gov/pmc/articles/PMC360971/
https://ncbi.nlm.nih.gov/pubmed/2304458
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