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Stable transfection of the human parasite Leishmania major delineates a 30-kilobase region sufficient for extrachromosomal replication and expression.

To delineate segments of the genome of the human protozoan parasite Leishmania major necessary for replication and expression, we developed a vector (pR-NEO) which can be reproducibly introduced into L. major. This DNA was derived from a 30-kilobase extrachromosomal amplified DNA bearing the dihydro...

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Vydáno v:Mol Cell Biol
Hlavní autoři: Kapler, G M, Coburn, C M, Beverley, S M
Médium: Artigo
Jazyk:Inglês
Vydáno: Taylor & Francis 1990
Témata:
On-line přístup:https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360971/
https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/2304458/
https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.10.3.1084
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