A sensitive real-time PCR based assay to estimate the impact of amino acid substitutions on the competitive replication fitness of human immunodeficiency virus type 1 in cell culture

Fixation of mutations in human immunodeficiency virus type 1 (HIV-1), such as those conferring drug resistance and immune escape, can result in a change in replication fitness. To assess these changes, a real-time TaqMan PCR detection assay and statistical methods for data analysis were developed to...

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Detalhes bibliográficos
Main Authors: Liu, Yi, Holte, Sarah, Rao, Ushnal, McClure, Jan, Konopa, Philip, Swain, J. Victor, Lanxon-Cookson, Erinn, Kim, Moon, Chen, Lennie, Mullins, James I.
Formato: Artigo
Idioma:Inglês
Publicado em: 2012
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Article
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3608845/
https://ncbi.nlm.nih.gov/pubmed/23201292
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2012.10.016
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