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Quantitation of HIV DNA integration: effects of differential integration site distributions on Alu-PCR assays

In many studies of HIV replication, it is useful to quantify the number of HIV proviruses in cells against a background of unintegrated forms of the HIV DNA. A popular method for doing so involves quantitative PCR using one primer complementary to the HIV long terminal repeat (LTR), and a second pri...

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Detalhes bibliográficos
Main Authors: Brady, Troy, Kelly, Brendan J., Male, Frances, Roth, Shoshannah, Bailey, Aubrey, Malani, Nirav, Gijbsers, Rik, O'Doherty, Una, Bushman, Frederic D.
Formato: Artigo
Idioma:Inglês
Publicado em: 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3608829/
https://ncbi.nlm.nih.gov/pubmed/23348058
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.jviromet.2013.01.004
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