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A Bead Aggregation Assay for Detection of Low-Affinity Protein-Protein Interactions Reveals Interactions between N-Terminal Domains of Inositol 1,4,5-Trisphosphate Receptors

Interactions between proteins are a hallmark of all cellular activities. Such interactions often occur with low affinity, a feature that allows them to be rapidly reversible, but it makes them difficult to detect using conventional methods such as yeast 2-hybrid analyses, co-immunoprecipitation or a...

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Detalhes bibliográficos
Main Authors: Chavda, Alap P., Prole, David L., Taylor, Colin W.
Formato: Artigo
Idioma:Inglês
Publicado em: Public Library of Science 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3608601/
https://ncbi.nlm.nih.gov/pubmed/23555994
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0060609
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