Cloning, expression, purification, crystallization and preliminary X-ray diffraction studies of N-acetylneuraminate lyase from methicillin-resistant Staphylococcus aureus

The enzyme N-acetylneuraminate lyase (EC 4.1.3.3) is involved in the metabolism of sialic acids. Specifically, the enzyme catalyzes the retro-aldol cleavage of N-acetylneuraminic acid to form N-acetyl-d-mannosamine and pyruvate. Sialic acids comprise a large family of nine-carbon amino sugars, all o...

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Hauptverfasser: North, Rachel A., Kessans, Sarah A., Atkinson, Sarah C., Suzuki, Hironori, Watson, Andrew J. A., Burgess, Benjamin R., Angley, Lauren M., Hudson, André O., Varsani, Arvind, Griffin, Michael D. W., Fairbanks, Antony J., Dobson, Renwick C. J.
Format: Artigo
Sprache:Inglês
Veröffentlicht: International Union of Crystallography 2013
Schlagworte:
Crystallization Communications
Online Zugang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3606580/
https://ncbi.nlm.nih.gov/pubmed/23519810
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1107/S1744309113003060
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