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Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)

Palmitoylation is a post-translational lipid modification involving the attachment of a 16-carbon saturated fatty acid, palmitate, to cysteine residues of substrate proteins through a labile thioester bond [reviewed in(1)]. Palmitoylation of a substrate protein increases its hydrophobicity, and typi...

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Detalhes bibliográficos
Main Authors: Brigidi, G. Stefano, Bamji, Shernaz X
Formato: Artigo
Idioma:Inglês
Publicado em: MyJove Corporation 2013
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605617/
https://ncbi.nlm.nih.gov/pubmed/23438969
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50031
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