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Detection of Protein Palmitoylation in Cultured Hippocampal Neurons by Immunoprecipitation and Acyl-Biotin Exchange (ABE)
Palmitoylation is a post-translational lipid modification involving the attachment of a 16-carbon saturated fatty acid, palmitate, to cysteine residues of substrate proteins through a labile thioester bond [reviewed in(1)]. Palmitoylation of a substrate protein increases its hydrophobicity, and typi...
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| Main Authors: | , |
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| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
MyJove Corporation
2013
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3605617/ https://ncbi.nlm.nih.gov/pubmed/23438969 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.3791/50031 |
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