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Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7

BACKGROUND: The hetero-hexamer of the eukaryotic minichromosome maintenance (MCM) proteins plays an essential role in replication of genomic DNA. The ring-shaped Mcm2-7 hexamers comprising one of each subunit show helicase activity in vitro, and form double-hexamers on DNA. The Mcm4/6/7 also forms a...

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Sonraí Bibleagrafaíochta
Main Authors: Xu, Meng, Chang, Y Paul, Chen, Xiaojiang S
Formáid: Artigo
Teanga:Inglês
Foilsithe: BioMed Central 2013
Ábhair:
Rochtain Ar Líne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605359/
https://ncbi.nlm.nih.gov/pubmed/23444842
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-14-5
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