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Expression, purification and biochemical characterization of Schizosaccharomyces pombe Mcm4, 6 and 7
BACKGROUND: The hetero-hexamer of the eukaryotic minichromosome maintenance (MCM) proteins plays an essential role in replication of genomic DNA. The ring-shaped Mcm2-7 hexamers comprising one of each subunit show helicase activity in vitro, and form double-hexamers on DNA. The Mcm4/6/7 also forms a...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
BioMed Central
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3605359/ https://ncbi.nlm.nih.gov/pubmed/23444842 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1471-2091-14-5 |
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