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An effective purification method using large bottles for human pancreatic islet isolation

The purification process is one of the most difficult procedures in pancreatic islet isolation. It was demonstrated that the standard purification method using a COBE 2991 cell processor with Ficoll density gradient solution harmed islets mechanically by high shear force. We reported that purificati...

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Hlavní autoři: Shimoda, Masayuki, Itoh, Takeshi, Iwahashi, Shuichi, Takita, Morihito, Sugimoto, Koji, Kanak, Mazhar A., Chujo, Daisuke, Naziruddin, Bashoo, Levy, Marlon F., Grayburn, Paul A., Matsumoto, Shinichi
Médium: Artigo
Jazyk:Inglês
Vydáno: Landes Bioscience 2012
Témata:
On-line přístup:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605168/
https://ncbi.nlm.nih.gov/pubmed/23221740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/isl.23008
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