An effective purification method using large bottles for human pancreatic islet isolation

The purification process is one of the most difficult procedures in pancreatic islet isolation. It was demonstrated that the standard purification method using a COBE 2991 cell processor with Ficoll density gradient solution harmed islets mechanically by high shear force. We reported that purificati...

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Detalhes bibliográficos
Main Authors: Shimoda, Masayuki, Itoh, Takeshi, Iwahashi, Shuichi, Takita, Morihito, Sugimoto, Koji, Kanak, Mazhar A., Chujo, Daisuke, Naziruddin, Bashoo, Levy, Marlon F., Grayburn, Paul A., Matsumoto, Shinichi
Formato: Artigo
Idioma:Inglês
Publicado em: Landes Bioscience 2012
Assuntos:
Research Paper
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3605168/
https://ncbi.nlm.nih.gov/pubmed/23221740
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.4161/isl.23008
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