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Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein.

The Saccharomyces cerevisiae SWI5 gene encodes a zinc finger protein required for the expression of the HO gene. A protein fusion between glutathione S-transferase and SWI5 was expressed in Escherichia coli and purified. The GST-SWI5 fusion protein formed only a low-affinity complex in vitro with th...

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Bibliografiske detaljer
Main Authors: Brazas, R M, Stillman, D J
Format: Artigo
Sprog:Inglês
Udgivet: 1993
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC360269/
https://ncbi.nlm.nih.gov/pubmed/8355698
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