Identification and purification of a protein that binds DNA cooperatively with the yeast SWI5 protein.
The Saccharomyces cerevisiae SWI5 gene encodes a zinc finger protein required for the expression of the HO gene. A protein fusion between glutathione S-transferase and SWI5 was expressed in Escherichia coli and purified. The GST-SWI5 fusion protein formed only a low-affinity complex in vitro with th...
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| Udgivet i: | Mol Cell Biol |
|---|---|
| Principais autores: | , |
| Format: | Artigo |
| Sprog: | Inglês |
| Udgivet: |
Taylor & Francis
1993
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| Fag: | |
| Online adgang: | https://ncbi.nlm.nih.govhttps://pmc.ncbi.nlm.nih.gov/articles/PMC360269/ https://ncbi.nlm.nih.govhttps://pubmed.ncbi.nlm.nih.gov/8355698/ https://ncbi.nlm.nih.govhttps://doi.org/10.1128/mcb.13.9.5524-5537.1993 |
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