Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures

BACKGROUND: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression levels. Validated normalization is essential to obtain reliable qPCR data. In that context, normalizing to multiple reference genes has become the most popular method. However, expression of refere...

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Bibliografiske detaljer
Main Authors: Swijsen, Ann, Nelissen, Katherine, Janssen, Daniel, Rigo, Jean-Michel, Hoogland, Govert
Format: Artigo
Sprog:Inglês
Udgivet: BioMed Central 2012
Fag:
Research Article
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3598510/
https://ncbi.nlm.nih.gov/pubmed/23237195
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-685
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