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Validation of reference genes for quantitative real-time PCR studies in the dentate gyrus after experimental febrile seizures
BACKGROUND: Quantitative real-time PCR (qPCR) is a commonly used technique to quantify gene expression levels. Validated normalization is essential to obtain reliable qPCR data. In that context, normalizing to multiple reference genes has become the most popular method. However, expression of refere...
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| Hauptverfasser: | , , , , |
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| Format: | Artigo |
| Sprache: | Inglês |
| Veröffentlicht: |
BioMed Central
2012
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| Schlagworte: | |
| Online Zugang: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3598510/ https://ncbi.nlm.nih.gov/pubmed/23237195 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1756-0500-5-685 |
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