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OPTIMIZATION OF A FILTER-LYSIS PROTOCOL TO PURIFY RAT TESTICULAR HOMOGENATES FOR AUTOMATED SPERMATID COUNTING
OBJECTIVES: Quantifying testicular homogenization resistant spermatid heads (HRSH) is a powerful indicator of spermatogenesis. These counts have traditionally been performed manually using a hemocytometer, but this method can be time consuming and biased. We aimed to develop a protocol to reduce deb...
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| Main Authors: | , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2012
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3598013/ https://ncbi.nlm.nih.gov/pubmed/22240558 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2164/jandrol.111.015131 |
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