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OPTIMIZATION OF A FILTER-LYSIS PROTOCOL TO PURIFY RAT TESTICULAR HOMOGENATES FOR AUTOMATED SPERMATID COUNTING

OBJECTIVES: Quantifying testicular homogenization resistant spermatid heads (HRSH) is a powerful indicator of spermatogenesis. These counts have traditionally been performed manually using a hemocytometer, but this method can be time consuming and biased. We aimed to develop a protocol to reduce deb...

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Bibliografiske detaljer
Main Authors: Pacheco, Sara E., Anderson, Linnea M., Boekelheide, Kim
Format: Artigo
Sprog:Inglês
Udgivet: 2012
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3598013/
https://ncbi.nlm.nih.gov/pubmed/22240558
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.2164/jandrol.111.015131
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