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Catalysis by N-Acetyl-d-glucosaminylphosphatidylinositol De-N-acetylase (PIG-L) from Entamoeba histolytica: NEW ROLES FOR CONSERVED RESIDUES

We showed previously that Entamoeba histolytica PIG-L exhibits a novel metal-independent albeit metal-stimulated activity. Using mutational and biochemical analysis, here we identify Asp-46 and His-140 of the enzyme as being important for catalysis. We show that these mutations neither affect the gl...

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Autori principali: Ashraf, Mohammad, Sreejith, Perinthottathil, Yadav, Usha, Komath, Sneha Sudha
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Biochemistry and Molecular Biology 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3597800/
https://ncbi.nlm.nih.gov/pubmed/23341455
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1074/jbc.M112.427245
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