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An efficient method for genome-wide polyadenylation site mapping and RNA quantification

The use of alternative poly(A) sites is common and affects the post-transcriptional fate of mRNA, including its stability, subcellular localization and translation. Here, we present a method to identify poly(A) sites in a genome-wide and strand-specific manner. This method, termed 3′T-fill, initiall...

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Détails bibliographiques
Auteurs principaux: Wilkening, Stefan, Pelechano, Vicent, Järvelin, Aino I., Tekkedil, Manu M., Anders, Simon, Benes, Vladimir, Steinmetz, Lars M.
Format: Artigo
Langue:Inglês
Publié: Oxford University Press 2013
Sujets:
Accès en ligne:https://ncbi.nlm.nih.gov/pmc/articles/PMC3597643/
https://ncbi.nlm.nih.gov/pubmed/23295673
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1093/nar/gks1249
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