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Directed Construction and Analysis of a Sinorhizobium meliloti pSymA Deletion Mutant Library

Resources from the Sinorhizobium meliloti Rm1021 open reading frame (ORF) plasmid libraries were used in a medium-throughput method to construct a set of 50 overlapping deletion mutants covering all of the Rm1021 pSymA megaplasmid except the replicon region. Each resulting pSymA derivative carried a...

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Bibliografiske detaljer
Main Authors: Yurgel, Svetlana N., Mortimer, Michael W., Rice, Jennifer T., Humann, Jodi L., Kahn, Michael L.
Format: Artigo
Sprog:Inglês
Udgivet: American Society for Microbiology 2013
Fag:
Online adgang:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592244/
https://ncbi.nlm.nih.gov/pubmed/23335760
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.02974-12
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