Construction and Expression of Sugar Kinase Transcriptional Gene Fusions by Using the Sinorhizobium meliloti ORFeome

The Sinorhizobium meliloti ORFeome project cloned 6,314 open reading frames (ORFs) into a modified Gateway entry vector system from which the ORFs could be transferred to destination vectors in vivo via bacterial conjugation. In this work, a reporter gene destination vector, pMK2030, was constructed...

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Detalhes bibliográficos
Main Authors: Humann, Jodi L., Schroeder, Brenda K., Mortimer, Michael W., House, Brent L., Yurgel, Svetlana N., Maloney, Scott C., Ward, Kristel L., Fallquist, Heather M., Ziemkiewicz, Hope T., Kahn, Michael L.
Formato: Artigo
Idioma:Inglês
Publicado em: American Society for Microbiology (ASM) 2008
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Methods
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC2576705/
https://ncbi.nlm.nih.gov/pubmed/18791020
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.01468-08
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