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Isolation of a Substantial Proportion of Forest Soil Bacterial Communities Detected via Pyrotag Sequencing

We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indi...

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Autors principals: VanInsberghe, David, Hartmann, Martin, Stewart, Gordon R., Mohn, William W.
Format: Artigo
Idioma:Inglês
Publicat: American Society for Microbiology 2013
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592214/
https://ncbi.nlm.nih.gov/pubmed/23315727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03112-12
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