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Isolation of a Substantial Proportion of Forest Soil Bacterial Communities Detected via Pyrotag Sequencing

We isolated 1,264 bacterial strains from forest soils previously surveyed via pyrosequencing of rRNA gene amplicons. Conventional culturing techniques recovered a substantial proportion of the community, with isolates representing 22% of 98,557 total pyrotags. Growth characteristics of isolates indi...

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Autori principali: VanInsberghe, David, Hartmann, Martin, Stewart, Gordon R., Mohn, William W.
Natura: Artigo
Lingua:Inglês
Pubblicazione: American Society for Microbiology 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3592214/
https://ncbi.nlm.nih.gov/pubmed/23315727
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1128/AEM.03112-12
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