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Molecular deconvolution of the monoclonal antibodies that comprise the polyclonal serum response

We have developed and validated a methodology for determining the antibody composition of the polyclonal serum response after immunization. Pepsin-digested serum IgGs were subjected to standard antigen-affinity chromatography, and resulting elution, wash, and flow-through fractions were analyzed by...

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Detalhes bibliográficos
Main Authors: Wine, Yariv, Boutz, Daniel R., Lavinder, Jason J., Miklos, Aleksandr E., Hughes, Randall A., Hoi, Kam Hon, Jung, Sang Taek, Horton, Andrew P., Murrin, Ellen M., Ellington, Andrew D., Marcotte, Edward M., Georgiou, George
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2013
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Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3581903/
https://ncbi.nlm.nih.gov/pubmed/23382245
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1213737110
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