Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1

Aldehyde dehydrogenase 3A1 (ALDH3A1) is a recently characterized corneal crystallin with its exact functions still being unclear. Expressing recombinant human ALDH3A1 has been difficult in Escherichia coli (E. coli) because of low solubility, yield and insufficient purity issues. In this report, we...

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Autori principali: Voulgaridou, Georgia-Persephoni, Mantso, Theodora, Chlichlia, Katerina, Panayiotidis, Mihalis I., Pappa, Aglaia
Natura: Artigo
Lingua:Inglês
Pubblicazione: Public Library of Science 2013
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Research Article
Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3579847/
https://ncbi.nlm.nih.gov/pubmed/23451057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056582
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