Efficient E. coli Expression Strategies for Production of Soluble Human Crystallin ALDH3A1

Aldehyde dehydrogenase 3A1 (ALDH3A1) is a recently characterized corneal crystallin with its exact functions still being unclear. Expressing recombinant human ALDH3A1 has been difficult in Escherichia coli (E. coli) because of low solubility, yield and insufficient purity issues. In this report, we...

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Autors principals: Voulgaridou, Georgia-Persephoni, Mantso, Theodora, Chlichlia, Katerina, Panayiotidis, Mihalis I., Pappa, Aglaia
Format: Artigo
Idioma:Inglês
Publicat: Public Library of Science 2013
Matèries:
Research Article
Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC3579847/
https://ncbi.nlm.nih.gov/pubmed/23451057
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0056582
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