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DNAzyme Footprinting: Detecting Protein-Aptamer Complexation on Surfaces by Blocking DNAzyme Cleavage Activity
A novel method to quantitatively measure the binding of 1proteins to single-stranded DNA (ssDNA) aptamers that employs the inhibition of the DNAzyme hydrolysis of aptamer monolayers is described. A 28-base DNAzyme was designed to specifically bind to and cleave a 29-base ssDNA sequence that can fold...
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| Autors principals: | , |
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| Format: | Artigo |
| Idioma: | Inglês |
| Publicat: |
2013
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| Matèries: | |
| Accés en línia: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3576845/ https://ncbi.nlm.nih.gov/pubmed/23351044 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/ja311367t |
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