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Live-Cell Superresolution Imaging by Pulsed STED Two-Photon Excitation Microscopy

Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of one-photon imaging approaches. The spatial resolution of conventional 2PLSM is limited by diffraction, and the ne...

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Autori principali: Takasaki, Kevin T., Ding, Jun B., Sabatini, Bernardo L.
Natura: Artigo
Lingua:Inglês
Pubblicazione: The Biophysical Society 2013
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Accesso online:https://ncbi.nlm.nih.gov/pmc/articles/PMC3576532/
https://ncbi.nlm.nih.gov/pubmed/23442955
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.12.053
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