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Live-Cell Superresolution Imaging by Pulsed STED Two-Photon Excitation Microscopy
Two-photon laser scanning microscopy (2PLSM) allows fluorescence imaging in thick biological samples where absorption and scattering typically degrade resolution and signal collection of one-photon imaging approaches. The spatial resolution of conventional 2PLSM is limited by diffraction, and the ne...
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| Main Authors: | , , |
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| Format: | Artigo |
| Language: | Inglês |
| Published: |
The Biophysical Society
2013
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| Subjects: | |
| Online Access: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3576532/ https://ncbi.nlm.nih.gov/pubmed/23442955 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.bpj.2012.12.053 |
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