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Single-wavelength two-photon excitation–stimulated emission depletion (SW2PE-STED) superresolution imaging

We developed a new class of two-photon excitation–stimulated emission depletion (2PE-STED) optical microscope. In this work, we show the opportunity to perform superresolved fluorescence imaging, exciting and stimulating the emission of a fluorophore by means of a single wavelength. We show that a w...

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Detalhes bibliográficos
Main Authors: Bianchini, Paolo, Harke, Benjamin, Galiani, Silvia, Vicidomini, Giuseppe, Diaspro, Alberto
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2012
Assuntos:
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3340040/
https://ncbi.nlm.nih.gov/pubmed/22493221
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.1119129109
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