Development of a sensitive RT-PCR method for amplifying and sequencing near full-length HCV genotype 1 RNA from patient samples

BACKGROUND: Direct-acting antiviral (DAAs) agents for hepatitis C virus (HCV) span a variety of targets, including proteins encoded by the NS3/4A, NS4B, NS5A, and NS5B genes. Treatment with DAAs has been shown to select variants with sequence changes in the HCV genome encoding amino acids that may c...

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Detalhes bibliográficos
Main Authors: Zhang, Eileen Z, Bartels, Doug J, Frantz, J Dan, Seepersaud, Sheila, Lippke, Judith A, Shames, Benjamin, Zhou, Yi, Lin, Chao, Kwong, Ann, Kieffer, Tara L
Formato: Artigo
Idioma:Inglês
Publicado em: BioMed Central 2013
Assuntos:
Methodology
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC3575352/
https://ncbi.nlm.nih.gov/pubmed/23402332
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1186/1743-422X-10-53
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