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Energetic coupling between an oxidizable cysteine and the phosphorylatable N-terminus of human liver pyruvate kinase()
During our efforts to characterize regulatory properties of human liver pyruvate kinase (L-PYK), we have noted that the affinity of the protein for PEP becomes reduced after several days post cell lysis. A 1.8Å crystallographic structure of L-PYK with the S12D mimic of phosphorylation indicates that...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2013
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3572911/ https://ncbi.nlm.nih.gov/pubmed/23270483 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1021/bi301341r |
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