Short interfering RNAs can induce unexpected and divergent changes in the levels of untargeted proteins in mammalian cells

RNA interference (RNAi) mediated by short interfering RNAs (siRNAs) is a widely used method to analyze gene function. To use RNAi knockdown accurately to infer gene function, it is essential to determine the specificity of siRNA-mediated RNAi. We have assessed the specificity of 10 different siRNAs...

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Detalhes bibliográficos
Main Authors: Scacheri, Peter C., Rozenblatt-Rosen, Orit, Caplen, Natasha J., Wolfsberg, Tyra G., Umayam, Lowell, Lee, Jeffrey C., Hughes, Christina M., Shanmugam, Kalai Selvi, Bhattacharjee, Arindam, Meyerson, Matthew, Collins, Francis S.
Formato: Artigo
Idioma:Inglês
Publicado em: National Academy of Sciences 2004
Assuntos:
Biological Sciences
Acesso em linha:https://ncbi.nlm.nih.gov/pmc/articles/PMC357023/
https://ncbi.nlm.nih.gov/pubmed/14769924
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1073/pnas.0308698100
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