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Solid-phase capture for the detection and relative quantification of S-nitrosoproteins by mass spectrometry
The proteomic analysis of S-nitrosylated protein (SNO-proteins) has long depended on the biotin switch technique (BST), which requires blocking of free thiols, ascorbate-based denitrosylation of SNO-Cys, biotinylation of nascent thiol and avidin-based affinity isolation. A more recent development is...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado: |
2012
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| Assuntos: | |
| Acceso en liña: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3568208/ https://ncbi.nlm.nih.gov/pubmed/23064468 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1016/j.ymeth.2012.10.001 |
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