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Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture
We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-...
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| Main Authors: | , , , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
2009
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC2891235/ https://ncbi.nlm.nih.gov/pubmed/19483679 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1545 |
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