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Proteomic analysis of S-nitrosylation and denitrosylation by resin-assisted capture

We have modified the biotin switch assay for protein S-nitrosothiols (SNOs), using resin-assisted capture (SNO-RAC). Compared with existing methodologies, SNO-RAC requires fewer steps, detects high-mass S-nitrosylated proteins more efficiently, and facilitates identification and quantification of S-...

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Autors principals: Forrester, Michael T, Thompson, J Will, Foster, Matthew W, Nogueira, Leonardo, Moseley, M Arthur, Stamler, Jonathan S
Format: Artigo
Idioma:Inglês
Publicat: 2009
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Accés en línia:https://ncbi.nlm.nih.gov/pmc/articles/PMC2891235/
https://ncbi.nlm.nih.gov/pubmed/19483679
https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1038/nbt.1545
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