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Production of Soluble Human Vascular Endothelial Growth Factor VEGF-A(165)-Heparin Binding Domain in Escherichia coli
We report a method for production of soluble heparin binding domain (HBD) of human vascular endothelial growth factor VEGF-A(165). Recombinant VEGF-A(165)-HBD that contains four disulphide bridges was expressed in specialised E. coli SHuffle cells and its activity has been confirmed through interact...
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| Main Authors: | , , , |
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| Formato: | Artigo |
| Idioma: | Inglês |
| Publicado em: |
Public Library of Science
2013
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| Assuntos: | |
| Acesso em linha: | https://ncbi.nlm.nih.gov/pmc/articles/PMC3568127/ https://ncbi.nlm.nih.gov/pubmed/23409021 https://ncbi.nlm.nih.govhttp://dx.doi.org/10.1371/journal.pone.0055690 |
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